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Ensure that all the purification buffers are at four °C and carry out the protein purification within the cold room to keep away from protein denaturation. WORD: This avoids the coupling of amino acids in the further cycles with the membrane instead of the growing peptide chain. WORD: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and also among the amino teams inside the spot areas do not kind bonds with the amino acids.
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Frequent posttranslational modifications include methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter happens in calmodulin Other posttranslational modifications at lysine residues embrace acetylation , sumoylation , and ubiquitination Collagen comprises hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues within the endoplasmic reticulum or Golgi equipment is used to mark sure proteins for secretion from the cell In opsins like rhodopsin and the visible opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde kinds a Schiff base with a conserved lysine residue, and interaction of sunshine with the retinylidene group causes sign transduction in color imaginative and prescient (See visual cycle for particulars).
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